Transcriptomic analysis of osteosarcoma data with HISAT2 and StringTie software.
Julia Pietro and Jo to Meidanis.
April 2022. In English, 12 pages.

Summary: Transcriptomic analyzes have been shown to be necessary in the field of Medicine, as it is possible to identify DNA transcription products in different molecules. In the case of mutations, as in cancer cells, transcripts and proteins undergo changes, which makes it possible to analyze the expression pattern, based on the products and active pathways. From this, treatments can be developed, aiming at the survival and well-being of patients. Thus, based on data from 35 patients from the Boldrini Children's Center and with the help of CENAPAD, RNA sequences from children with osteosarcoma were analyzed via software and programming languages. The main objective of this project was to obtain two final products: matrices with gene counters and sample transcripts, which will feed studies on metabolic pathways and differential gene expression. For this, the samples underwent quality tests, sequence cleaning, mapping with a reference genome and, finally, the transcript counting process. The reports before the cleanup expressed results with low base statistics. These percentages helped to make the necessary adjustments to the samples, such as removing low quality bases. Thus, right after cleaning the data, the quality report showed significant improvements in the quality of the sequences. Then, mapping the reads with the reference genome resulted in an alignment rate with an average of 95,91%. Finally, transcript counts provided tables with levels of transcripts and genes expressed by samples. In this way, during PEOp 2022 it was possible to develop bioinformatics techniques and reach the expected result.

Virtual watercolor: Investigating imagination in a socioenactive experience.
Maria Jêsca Nobre de Queiroz, Josiane Rosa de Oliveira Gaia Pimenta, Emanuel Felipe Duarte, Yusseli Lizeth Méndez Mendoza, Julio Cesar dos Reis, and M. Cecília C. Baranauskas.
March 2022. In Portuguese, 14 pages.

Abstract: This technical report describes the research activities that investigate the imagination within the context of using the Aquarela Virtual system. This study was conducted during the fifth year of the Thematic Project Socioenactive Systems (Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, #2015/16528-0). In this report, we briefly present the context of the Projeto Sistemas Socioenativas, we describe the concept of imagination; then we describe the concept of the Aquarela Virtual system, motivated by the social isolation resulting from the COVID-19 pandemic. In particular, we present preliminary results of a case study on the use of imagination in the interaction with the Aquarela Virtual system.

Virtual watercolor: Investigating equitable access in a socio-enactive facility in a context of social isolation.
Josiane Rosa de Oliveira Gaia Pimenta, Emanuel Felipe Duarte, Maria Jêsca Nobre de Queiroz, Yusseli Lizeth Méndez Mendoza, José Valderlei da Silva, and M. Cecília C. Baranauskas.
February 2022. In Portuguese, 16 pages.

Summary: This technical report describes the equitable access assessment actions in the Aquarela Virtual workshops, a system developed during the fifth year of the Socioenativos Systems Thematic Project (Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, #2015/16528-0). Aquarela Virtual is an initiative to study socioenative systems in the remote context generated by the social isolation of the COVID-19 pandemic. This report presents the workshop from an access perspective, as well as describes the facility assessment activities carried out and their preliminary results.

Preliminary results of an enactive socio-affective experience: A case study with the virtual watercolor system.
Luã Marcelo Muriana, Josiane Rosa de O. Gaia Pimenta, Maria Jêsca Nobre de Queiroz, Yusseli Lizeth Méndez Mendoza, and M. Cecília C. Baranauskas.
February 2022. In Portuguese, 16 pages.

Summary: Cognition, from the perspective of enaction, is a vital, embodied, creative and collective process, so that it acts as an embodied action. In this sense, the process of production of meaning (sense-making) occurs from the relationship between a subject and a world from the action-perception system of that subject. However, social relationships and emotional factors also play an active role in this process of production of meaning. Thus, the objective of this work is to analyze the socio-enactive experience during the interaction of children with the Virtual Aquarela System, highlighting the socio-emotional aspects of this process and how they affect the users' production of meaning (individually and socially). The preliminary results of the analysis highlight that even a virtual environment with distant users and with remote access (without physical presence), has the capacity to influence the emotional socio-affectivity between them.

Virtual watercolor: Design and development of a socioenactive system in a context of social isolation.
Emanuel Felipe Duarte, Yusseli Lizeth Méndez Mendoza, Maria Jêsca Nobre de Queiroz, Josiane Rosa de Oliveira Gaia Pimenta, José Valderlei da Silva, and M. Cecília C. Baranauskas.
February 2022. In Portuguese, 20 pages.

Summary: This technical report presents the process of design and development of the Aquarela Virtual system, carried out during the fifth year of the Thematic Project Socioenactive Systems (Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, #2015/16528-0). In this report, we briefly present the context of the Socio-enactive Systems Thematic Project, and then we describe the concept of the Virtual Aquarela system, motivated by the social isolation resulting from the COVID-19 pandemic. The main functionalities of the system are described and discussed in this report, with the aim of documenting design and development decisions. Finally, we end the report with conclusions about the process and some possible directions for system improvements over the next year.