LOCUS HUMPSAP 4778 bp DNA PRI 15-MAR-1990 DEFINITION Human pulmonary surfactant apoprotein (PSAP) gene, complete cds. ACCESSION M30838 NID g190564 KEYWORDS pulmonary surfactant protein. SOURCE Human adult lung DNA, adult lung, cDNA to mRNA, clones HS [2 and 5] and fetal lung, cDNA to mRNA, clone pHS-6. ORGANISM Homo sapiens Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata; Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 4778) AUTHORS White,R.T., Damm,D.L., Miller,J., Spratt,K., Schilling,J., Hawgood,A., Benson,B.J. and Cordell,B. TITLE Isolation and characterization of the human pulmonary surfactant apoprotein gene JOURNAL Nature 317, 361-363 (1985) MEDLINE 86014366 FEATURES Location/Qualifiers source 1..4778 /organism="Homo sapiens" /db_xref="taxon:9606" TATA_signal 128..133 prim_transcript 171..4637 /note="PSAP mRNA and introns" intron 200..1022 /note="PSAP intron A" sig_peptide 1046..1105 /note="pulmonary surfactant apoprotein signal peptide" CDS join(1046..1217,1531..1650,2404..2481,2957..3333) /note="pulmonary surfactant apoprotein precursor" /codon_start=1 /db_xref="PID:g190565" /translation="MWLCPLALNLILMAASGAVCEVKDVCVGSPGIPGTPGSHGLPGR HGRDGLKGDLGPPGPMGPPGEMPCPPGNDGLPGAPGIPGECGEKGEPGERGPPGLRAH LDEELQATLHDFRHQILQTRGALSLQGSIMTVGEKVFSSNGQSITFDAIQEACARAGG RIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAG RGKEQCVEMYTDGQWNDRNCLYSRLTICEF" exon <1046..1217 /note="pulmonary surfactant apoprotein precursor, (first expressed exon)" /number=2 mat_peptide 1106..1217 /note="pulmonary surfactant apoprotein" intron 1218..1530 /note="PSAP intron B" mat_peptide 1531..1650 /note="pulmonary surfactant apoprotein" exon 1531..1650 /number=3 intron 1651..2403 /note="PSAP intron C" mat_peptide 2404..2481 /note="pulmonary surfactant apoprotein" exon 2404..2481 /number=4 intron 2482..2956 /note="PSAP intron D" exon 2957..>3333 /note="pulmonary surfactant apoprotein precursor" /number=5 mat_peptide 2957..3330 /note="pulmonary surfactant apoprotein" polyA_signal 4619..4624 polyA_signal 4639..4644 BASE COUNT 1152 a 1223 c 1350 g 1053 t ORIGIN 1 bp upstream of BamHI site. 1 ggatcctcca gcctgagtgc tcttggggaa acatgctgtg taaacactat gcccatttcc 61 tgcctggagc acaggttttg tggtagggct ctcaggggtg aggaggaagc ctggcagccc 121 ccacatctat aaatgctgcg tctaccttac cctctgactt ggaggcagag acccaagcag 181 ctggaggctc tgtgtgtggg tgagtttagc cccatcccct aggtgttctc cagcttgagg 241 atcgcaggca gagaggacca gcccagcagc cacaggcctg accaaagccc aggctgggaa 301 ggagggcaac tccccatttt ccactgggag gtgtttcaca gcacagtcaa cataggtgac 361 ctgcaaagat cctcatgttt gttattttct ttggccagat ccatcctaca gggttcagca 421 gggcctacag gaggggcagt gagagaacag accccaaaaa gaaaggggac tccatgactg 481 accaccttga ggggggccag gctgccgggc cccgttcatc ttttttcatt ctcaggtcgc 541 tgatttcttg gagcctgaaa agaaagtaac acagcaggga tgaggacaga tggtgtgagt 601 cagtgagtga gtgacctgac taatagcctg ggagggacag ggcaggtttt ctgcagaggc 661 acggaagatt cagctgaagt cagagaggtg aagccagttt cccagggtaa catagtgagg 721 cactgaaaga aaggagactg cactggagcc caggtccccg ggctccccag agctccttac 781 tcttcctcct cctcagcagc ctggagaccc cacaacctcc agccggaggc ctgaagcatg 841 aggccatgcc aggtgccagg tgatgctggg aattttcccg ggagcttcgg gtcttcccag 901 cactctggtc gtcgcccgcc ctgcctcgtc gggctctgcc cagcttcctg agtcctgaca 961 gagcacagtg ggggagatgt tggcagaggt ggcagatggg ctcacggcca tccctcctgc 1021 aggagcagcg actggaccca gagccatgtg gctgtgccct ctggccctca acctcatctt 1081 gatggcagcc tctggtgctg tgtgcgaagt gaaggacgtt tgtgttggaa gccctggtat 1141 ccccggcact cctggatccc acggcctgcc aggcaggcac gggagagatg gtctcaaagg 1201 agacctgggc cctccaggta ctgtgctgca gaccccaccc tcagctgagg acacagaccc 1261 cttttcagga ggcccatctg tccaggcccc taggctgtgg gccatagtga gctgggggct 1321 atagtaagct gggtgggact tcagtctgca gggctggtgg gttcctgggg cccttatgat 1381 ggcgcatcct ggagagtctg tcctcatagt gcccacggag tgatagagtg atagctgagc 1441 cagccctggt gataatgggc atcgagtctc actagctcca accagttgtg ggtgacagat 1501 cctacacatc catgtctctt ttctctgcag gccccatggg tccacctgga gaaatgccat 1561 gtcctcctgg aaatgatggg ctgcctggag cccctggtat ccctggagag tgtggagaga 1621 agggggagcc tggcgagagg ggccctccag gtgagcaggg tggggcaggt gggcagtgga 1681 aacatgggca cagcgaccct gaagtcagtt acacggggat gatggggatc agacaaaccc 1741 tacaggttcc ccaagggcat ttggctcaac ctaagtaaga gaggataagc ttgagggaga 1801 tagctgaggt gtctggggag tgtggtcaca attcagggaa aggcaggtgt gggaagtcct 1861 ccgtgcctca tgaccaccga tggggacaca ctgagtcagg tgtgggatga gggacagcac 1921 tgggaggcag gggaggcatg tcctgggatg gaggccctgg ggctgtctga agggtgaatg 1981 cggacgaggc atccagacag acggtgtgat caggagcccc acagacagag gggaactttg 2041 aagctcagag cggtaagcaa gtccatcagg gcagtgcaga gagcatcatg cttgcccttc 2101 ggtcggaggg tgcgggagag ggacttgccc cacagaggcg ggcagacaga acccctcgag 2161 gacaagagca ggaaagagga caaggggtgg gggtctcagc aggggcaagg cttcactaaa 2221 gaatagggga ccacgggtct gagacacact ggaatcttgt ggaccctctg agcctaggtc 2281 tggtggcgcc taacagcaat gaaagggcag agttccagga ttgcagatgg caaaacacct 2341 cgtggcagca agtgggagtc ttcactggcc tgcccctcct tctgtgtggg gcactctcca 2401 cagggcttcg agctcatcta gatgaggagc tccaagccac actccacgac tttagacatc 2461 aaatcctgca gacaagggga ggtaagggga ccccctgggc tcacggggta ggagtttccc 2521 acaaattccc ctcattctca gcaccagctt ctagaacata gagattacaa ataggcatgc 2581 acatgcaggt cttggggaaa ggaattgacg cttgcttttc ttgatgtctt ttgaatggcc 2641 cagaggagac agaagcagac acaattcact tccccgattt cataggaaag caagttctct 2701 atctgccttg ctttccactg aattcacagg aaattgcacc atttctggca ataagtaatt 2761 gttacttagg tgaatgaata aatggaggag agtctaaaag tgaatttaga aaactgcaat 2821 tggaagagga agagaagaca cagagagagg cagagatgga gagactgggg agaatctggt 2881 agcagagacc ccaggtgagg gaggtggctt agagacaaag tggtcagtgg cctgacccgg 2941 actcctctgc tctcagccct cagtctgcag ggctccataa tgacagtagg agagaaggtc 3001 ttctccagca atgggcagtc catcactttt gatgccattc aggaggcatg tgccagagca 3061 ggcggccgca ttgctgtccc aaggaatcca gaggaaaatg aggccattgc aagcttcgtg 3121 aagaagtaca acacatatgc ctatgtaggc ctgactgagg gtcccagccc tggagacttc 3181 cgctactcag acggcacccc tgtaaactac accaactggt accgagggga gcccgcaggt 3241 cggggaaaag agcagtgtgt ggagatgtac acagatgggc agtggaatga caggaactgc 3301 ctgtactccc gactgaccat ctgtgagttc tgagaggcat ttaggccatg ggacagggag 3361 gacgctctcc ttgtcggcct ccatcctgag gctccacttg gtctgtgaga tgctagaact 3421 ccctttcaac agaattccac ttgtggctat tgggactgga ggcaccctta gccacttcat 3481 tcctctgatg ggccctgact cttccccata atcactcgac cagccttgac actccccttg 3541 caaactctcc cagcactgca ccccaggcag ccactcttag ccttggcctt cgacatgaga 3601 tggagccctc cttattcccc atctggtcca gttccttcac ttacagatgg cagcagtgag 3661 gtcttggggt agaaggaccc tccaaagtca cacaaagtgc ctgcctcctg gtcccctcag 3721 ctctctctct gcaacccagt gccatcagga tgagcaatcc tggccaagca taatgacaga 3781 gagaggcaga cttcggggaa gccctgactg tgcagagcta aggacacagt ggagattctc 3841 tggcactctg aggtctctgt ggcaggcctg gtcaggctct ccatgaggtt agaaggccag 3901 gtagttgttc cagcagggtg gtggccaagc caaccccatg attgatgtgt acgattcact 3961 cctttgagtc tttgaatggc aactcagccc cctgacctga agacagccag cctaggcctc 4021 taggtgacct agagccgcct tcagatgtga cccgagtaac tttcaactga tgaacaaatc 4081 tgcaccctac ttcagatttc agtgggcatt cacatcaccc ccacaccact ggctctgctt 4141 tctcctttca ttaatccatt cacccagata tttcattaaa attatcacgt gccaggtctt 4201 aggatatgtc gtggggtggg caaggtaatc agtgacagtt gaagattttt ttttcccaga 4261 gcttatgtct tcatctgtga aatgggaata agatacttgt tgctgtcaca gttattacca 4321 tccccccagc taccaaaatt actaccagaa ctgttactat acacagaggc tattgactga 4381 gcacctatca tttgccaaga accttgacaa gcacttctaa tacagcatat tatgtactat 4441 tcaatcttca cacaatgtca cgggaccagt attgtttcct cattttttat aaggacactg 4501 aagcttggag gagttaaatg ttttgagtat tattccagag agcaagtggc agaggctgga 4561 tccaaaccca tcttcctgga cctgaagctt atgcttccag cctcccactc ctgagctgaa 4621 taaagatgat ttaagcttaa taaatcgtga atgtgttcac atgagtttcc atagctttgg 4681 ttccaagaaa tatcacattt ctgtattttt gtaaatcaaa tgaactctga ctctgagccc 4741 cacttgcctg aagattggaa attcaatctc aggatgtg //